Simple flow microphotometer for rapid cell population analysis
- 1 August 1975
- journal article
- research article
- Published by AIP Publishing in Review of Scientific Instruments
- Vol. 46 (8) , 1021-1024
- https://doi.org/10.1063/1.1134399
Abstract
A simple particle analyzer has been developed using a modified high speed flow‐through technique and absorption measurements of a continuous He–Ne laser beam. From pulse‐shape analysis and the integration of absorption pulses, size distributions of stained or unstained cells can be derived. The range of 5–300 μ is covered without changing the flow system and a rate up to 200 000/sec can be achieved. Typical applications in cell analyses are shown.Keywords
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