Abstract
Summary Extracellular pH in the range 5.5 to 8.0 affects the yields of Herpes simplex virus obtained after one reproductive cycle in HEp-2 cells. For 3 strains of virus the extracellular pH for maximum yields are 6.5–6.7, 7.0, and 7.2–7.3, respectively. The data show that (a) extracellular pH for optimal virus multiplication is determined by the genetic constitution of the virus, and (b) HEp-2 cells support viral macromolecular synthesis when incubated in a medium buffered at pH as low as 6.0, i.e., below the pH range for optimal cell multiplication and maintenance.

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