Cellulose Synthase-Like CslF Genes Mediate the Synthesis of Cell Wall (1,3;1,4)-ß- d -Glucans
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- 31 March 2006
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 311 (5769) , 1940-1942
- https://doi.org/10.1126/science.1122975
Abstract
A characteristic feature of grasses and commercially important cereals is the presence of (1,3;1,4)-β- d -glucans in their cell walls. We have used comparative genomics to link a major quantitative trait locus for (1,3;1,4)-β- d -glucan content in barley grain to a cluster of cellulose synthase–like CslF genes in rice. After insertion of rice CslF genes into Arabidopsis , we detected (1,3;1,4)-β- d -glucan in walls of transgenic plants using specific monoclonal antibodies and enzymatic analysis. Because wild-type Arabidopsis does not contain CslF genes or have (1,3;1,4)-β- d -glucans in its walls, these experiments provide direct, gain-of-function evidence for the participation of rice CslF genes in (1,3;1,4)-β- d -glucan biosynthesis.Keywords
This publication has 18 references indexed in Scilit:
- (1→3),(1→4)‐ß‐d‐Glucans in the cell walls of the Poales (sensu lato): an immunogold labeling study using a monoclonal antibodyAmerican Journal of Botany, 2005
- Crop biotechnology provides an opportunity to develop a sustainable futureTrends in Biotechnology, 2005
- Toward a Systems Approach to Understanding Plant Cell WallsScience, 2004
- The CesA Gene Family of Barley. Quantitative Analysis of Transcripts Reveals Two Groups of Co-Expressed GenesPlant Physiology, 2004
- Cellulose Synthase-Like Genes of RicePlant Physiology, 2002
- Mutant barley (1→3,1→4)-β-glucan endohydrolases with enhanced thermostabilityProtein Engineering, Design and Selection, 2001
- Multiple Cellulose Synthase Catalytic Subunits Are Required for Cellulose Synthesis in ArabidopsisPlant Cell, 2000
- Characterization of the Cell-Wall Polysaccharides of Arabidopsis thaliana LeavesPlant Physiology, 1995
- A (1→3,1→4)‐β‐glucan‐specific monoclonal antibody and its use in the quantitation and immunocytochemical location of (1→3,1→4)‐β‐glucansThe Plant Journal, 1994
- Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growthThe Plant Journal, 1993