Cloning of a human complement component C4 gene.

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Abstract
Six overlapping cosmid clones having an average insert size of 40 kilobase pairs were identified and isolated from a human genomic library by using a cDNA probe [complementary DNA], Alu-7, specific for the amino acid sequence of C4d, a known region of human complement component C4. Analysis of these genomic clones by restriction digestion and Southern blotting shows that all 6 probably contain the same complete C4 gene. Nucleotide sequence comparison of the genomic clone Cos-A and the cDNA clone Alu-7 shows an identical sequence except for the presence of a 1500-base pair intron in the genomic sequence. The amino acid sequence predicted from the nucleotide sequence agrees with the known C4d region amino acid sequence with 1 exception.