Immunochemical characterization and serum concentrations of pregnancy-associated serum proteins in mice

Abstract
The glycoprotein structure of 2 pregnancy-associated murine proteins, .alpha.1-pyridoxamine phosphate [PAMP] and .alpha.2-PAMP, was analyzed by using concanavalin A (Con A) affinity electrophoresis; .alpha.1-PAMP was completely precipitated by free Con A, whereas .alpha.2-PAMP showed heterogeneity. The isoelectric points of .alpha.1-PAMP and .alpha.2-PAMP were 4.2 and 4.0, respectively, using crossed immunoelectrofocusing. The .alpha.2-PAMP was detectable only in the serum of pregnant mice and fetuses. Maternal serum concentrations increased from day 8 of pregnancy, remained at high values from days 14-18, and could not be detected by 2 days post partum.

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