Single Radial Immunodiffusion Analysis for Quantitation of Colostral Immunoglobulin Concentration

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Abstract
Relative accuracy of the single radial immunodiffusion technique to measure Ig concentration of [cow] colostral preparations (whey, whole or fat-free) was assessed. Fresh colostrum samples were analyzed for major constituents. Gammaglobulin as a standard was compared to total Ig concentration derived from single radial immunodiffusion analysis of colostral preparations with no differences except between standard and whey. Differences were in part from enhancement or interference of Ig diffusion by colostral constituents. Removal of casein and fat during whey preparation caused a concentrating effect on Ig constituents resulting in exaggerated precipitin rings. Whey produced unreliable results; whole colostrum is recommended for single radial immunodiffusion analysis.