Short Communication:Maintenance of Glycoprotein-Determined Phenotype in an HIV Type 1 (pNL43)envGene-Cassetting System
- 1 November 2001
- journal article
- research article
- Published by Mary Ann Liebert Inc in AIDS Research and Human Retroviruses
- Vol. 17 (16) , 1501-1506
- https://doi.org/10.1089/08892220152644205
Abstract
Here we report the construction and use of a full-length env gene-cassetting system, C2, based on the HIV-1 infectious molecular clone NL43. C2 produces virus with the same phenotype as NL43 but with 2-fold lower growth kinetics. The latter probably relates to alteration in the vpu and/or nef genes. C2-env chimeras of macrophage-tropic and T cell-tropic laboratory strains and primary HIV-1 isolates retain the glycoprotein-determined phenotypes of their parent viruses. The cassette will assist studies of HIV-1 pathogenesis.Keywords
This publication has 44 references indexed in Scilit:
- HIV Type 1 Molecular Clones Able to Use the Bonzo/STRL-33 Coreceptor for Virus EntryAIDS Research and Human Retroviruses, 2001
- Human Immunodeficiency Virus Type 1 Subtype E Envelope Recombinant Peptides Containing Naturally Immunogenic EpitopesThe Journal of Infectious Diseases, 2000
- Partial “Repair” of DefectiveNEFGenes in a Long‐Term Nonprogressor with Human Immunodeficiency Virus Type 1 InfectionThe Journal of Infectious Diseases, 2000
- On the Role of the Second Coding Exon of the HIV-1 Tat Protein in Virus Replication and MHC Class I DownregulationAIDS Research and Human Retroviruses, 1998
- Interaction of the Second Coding Exon of Tat with Human EF-1δ Delineates a Mechanism for HIV-1-Mediated Shut-Off of Host mRNA TranslationBiochemical and Biophysical Research Communications, 1998
- HIV/SIV glycoproteins: structure-function relationshipsJournal of Molecular Biology, 1997
- A Leucine Zipper-Like Sequence from the Cytoplasmic Tail of the HIV-1 Envelope Glycoprotein Binds and Perturbs Lipid BilayersBiochemistry, 1997
- Identification of an ion channel activity of the Vpu transmembrane domain and its involvement in the regulation of virus release from HIV‐1‐infected cellsFEBS Letters, 1996
- An efficient method for the rescue and analysis of functional HIV-1 env genesAIDS, 1996
- Single amino-acid changes in HIV envelope affect viral tropism and receptor bindingNature, 1989