Improving the Enantioselectivity of theCandida CylindraceaLipase Via Chemical Modification
- 1 January 1992
- journal article
- research article
- Published by Taylor & Francis in Biocatalysis
- Vol. 6 (2) , 115-126
- https://doi.org/10.3109/10242429209014887
Abstract
The lipase of Candida cylindracea (B-form) was treated with the classical chemoselective reagents with a view to modifying the specific amino acid residue(s) in the protein. Nitration of the tyrosyl residues of this enzyme was achieved using an excess of tetranitromethane (TNM). This chemically modified TNM-lipase showed a remarkable improvement in enantioselectivity towards the hydrolysis of a series of aryloxypropionic and arylpropionic esters.Keywords
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