BCR‐ABL binds to IRS‐1 and IRS‐1 phosphorylation is inhibited by imatinib in K562 cells
- 23 December 2002
- journal article
- Published by Wiley in FEBS Letters
- Vol. 535 (1-3) , 17-22
- https://doi.org/10.1016/s0014-5793(02)03845-0
Abstract
In the present study we used K562 cells to demonstrate that insulin receptor substrate 1 (IRS-1) is expressed and constitutively phosphorylated in BCR-ABL(+) cells. We observed association between BCR-ABL/IRS-1, IRS-1/phosphoinositide 3'-kinase (PI3-kinase), and IRS-1/Grb2 in the K562 cell line. Our findings demonstrate that imatinib treatment resulted in marked attenuation of BCR-ABL/IRS-1 association and of IRS-1-stimulated PI3-kinase activity in K562 cells. We concluded that the IRS-1 protein is involved in the signalling pathway of the BCR-ABL tyrosine kinase.Keywords
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