Corticosteroid-Induced Differential Regulation of β-Adrenergic Receptors in Circulating Human Polymorphonuclear Leukocytes and Mononuclear Leukocytes*

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Abstract
A method of reproducibly measuring human leukocyte .beta.-adrenergic receptor density and affinity was developed and applied to the study of receptor regulation in man. The method has the advantages of using a membrane preparation which binds highly specifically and employing techniques such as using low concentrations of [3H]dihydroalprenolol, analyzing the data by computer modelling techniques and providing data from both granulocytes and lymphocytes in the same individual to minimize measurement errors. Using this methodology, human .beta.-adrenergic receptor regulation is examined. Cortisone acetate induced an acute rise in granulocyte .beta.-adrenergic receptor density and adenylate cyclase activity and an acute fall in lymphocyte .beta.-adrenergic receptor density. This potentially differential regulation of a single receptor subtype in 2 lines of leukocytes has important implications for the study of receptor regulation in man using leukocyte models.

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