Teflon-Sheathed, Fused Silica Connecting Tubing for Use in Microbore HPLC

1 July 1986

journal article
Published by Oxford University Press (OUP) in Journal of Chromatographic Science

Vol. 24 (7) , 285-288
https://doi.org/10.1093/chromsci/24.7.285

Abstract

Teflon-sheathed fused silica (TFS) tubing is examined as a viable alternative to stainless steel (s.s) tubing in microbore HPLC. Compared to 1.007-in. i.d s.s. tubing, a 4% to 39% (depending on k′) increase in plate count is found using TFS tubing of 10 cm × 0.0025 in. i.d. Dispersion measurements with this tubing in a columnless system show a total (“system”) variance of 4.5 μl², while the pressure drop is approximately 50 psi at 100 μlmin. It is shown that TFS tubing can be employed in a relatively long length (approximately 1 m) without introduced unacceptable extra-column disperation. Teflon-sheathed fused silica tubing is easy to handle and pressure stable up to 2500 psi.

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