Chlorophyll a/b-Binding Proteins, Pigment Conversions, and Early Light-Induced Proteins in a Chlorophyll b-less Barley Mutant
Open Access
- 1 March 1995
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 107 (3) , 873-883
- https://doi.org/10.1104/pp.107.3.873
Abstract
Monospecific polyclonal antibodies have been raised against synthetic peptides derived from the primary sequences from different plant light-harvesting Chl a/b-binding (LHC) proteins. Together with other monospecific antibodies, these were used to quantify the levels of the 10 different LHC proteins in wild-type and chlorina f2 barley (Hordeum vulgare L.), grown under normal and intermittent light (ImL). Chlorina f2, grown under normal light, lacked Lhcb1 (type I LHC II) and Lhcb6 (CP24) and had reduced amounts of Lhcb2, Lhcb3 (types II and III LHC II), and Lhcb4 (CP 29). Chlorina f2 grown under ImL lacked all LHC proteins, whereas wild-type ImL plants contained Lhcb5 (CP 26) and a small amount of Lhcb2. The Chlorina f2 ImL thylakoids were organized in large parallel arrays, but wild-type ImL thylakoids had appressed regions, indicating a possible role for Lhcb5 in grana stacking. Chlorina f2 grown under ImL contained considerable amounts of violaxanthin (2-3/reaction center), representing a pool of phototransformable xanthophyll cycle pigments not associated with LHC proteins. Chlorina f2 and the plants grown under ImL also contained early light-induced proteins (ELIPs) as monitored by western blotting. The levels of both ELIPs and xanthophyll cycle pigments increased during a 1 h of high light treatment, without accumulation of LHC proteins. These data are consistent with the hypothesis that ELIPs are pigment-binding proteins, and we suggest that ELIPs bind photoconvertible xanthophylls and replace “normal” LHC proteins under conditions of light stress.Keywords
This publication has 15 references indexed in Scilit:
- The light-harvesting chlorophyll ab-binding proteinsPublished by Elsevier ,2003
- The Effects of Illumination on the Xanthophyll Composition of the Photosystem II Light-Harvesting Complexes of Spinach Thylakoid MembranesPlant Physiology, 1994
- Appearance of Type 1, 2, and 3 Light-Harvesting Complex II and Light-Harvesting Complex I Proteins during Light-Induced Greening of Barley (Hordeum vulgare) EtioplastsPlant Physiology, 1994
- Cbr, an algal homolog of plant early light-induced proteins, is a putative zeaxanthin binding protein.Journal of Biological Chemistry, 1993
- Identification of the photosystem I antenna polypeptides in barleyEuropean Journal of Biochemistry, 1992
- Synthesis of the early light-inducible protein is controlled by blue light and related to light stress.Proceedings of the National Academy of Sciences, 1992
- Identification of type 1 and type 2 light-harvesting chlorophyll a/b-binding proteins using monospecific antibodiesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1992
- Biochemical composition and organization of higher plant photosystem II light-harvesting pigment-proteins.Journal of Biological Chemistry, 1991
- Chloroplasts of Arabidopsis thaliana homozygous for the ch-1 locus lack chlorophyll b, lack stable LHCPII and have stacked thylakoidsPlant Molecular Biology, 1991
- Chlorophyll regulates accumulation of the plastid-encoded chlorophyll apoproteins CP43 and D1 by increasing apoprotein stability.Proceedings of the National Academy of Sciences, 1990