Modulation of Angiotensin II Type 1 Receptor mRNA Expression in Human Blood Cells: Comparison of Platelets and Mononuclear Leucocytes.
- 1 January 1995
- journal article
- research article
- Published by Japan Endocrine Society in Endocrine Journal
- Vol. 42 (1) , 15-22
- https://doi.org/10.1507/endocrj.42.15
Abstract
The objective of the present study was to quantify the expression of angiotensin II type 1 (AT1) receptor transcripts in human blood cells-platelets and mononuclear leucocytes-from 10 normal healthy volunteers during the alterations in the renin-angiotensin system. A quantitative assay employing reverse transcription-polymerase chain reaction (RT-PCR) was utilized. Oral administration of furosemide, 40mg for 2 days, under mild salt restriction (50mEq NaCl/day) for 6 days stimulated the rennin-angiotensin system resulting in significant increases in plasma renin activity (PRA) (1.84±0.12vs. 1.05± 0.17ng/l/s; PPvs. 0.29±0.03) (Pvs. 0.25±0.05) (Pvs. 1.05±0.17ng/l/s; P<0.01) and induced the opposite changes in the ratio of AT1 receptor/GAPDH mRNA. These data suggest that AT1 receptors in human blood cells may be of two different types-platelets and mononuclear leucocytes.Keywords
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