Improved blocking of nonspecific antibody binding sites on nitrocellulose membranes
- 1 February 1984
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 5 (1) , 54-55
- https://doi.org/10.1002/elps.1150050110
Abstract
Nonspecific antibody binding either to nitrocellulose membrane sites or to transferred and immobilized proteins is eliminated (or minimized to insignificant levels) by treatment with a liquid gelatin preparation. This increases the specificity and sensitivity of nitrocellulose binding immunoassays, and allows incubation at 4°C which minimizes bacterial contamination and/or damage to biologically‐labile proteins.Keywords
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