Rapid extraction of high purity chromosomal DNA from Serratia marcescens
Open Access
- 1 February 1993
- journal article
- Published by Oxford University Press (OUP) in Letters in Applied Microbiology
- Vol. 16 (2) , 77-79
- https://doi.org/10.1111/j.1472-765x.1993.tb00348.x
Abstract
Rapid non-specific degradation of Serratia marcescens DNA extracted with guanidium thiocyanate, occurred within 10 min of incubation with restriction endo-nuclease enzymes. The described modified method based on chemical and enzymatic deproteinization produced preparations of Ser. marcescens DNA of high yield and quality which did not autodegrade when incubated with restriction endonucleases.Keywords
This publication has 2 references indexed in Scilit:
- Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogensJournal of Medical Microbiology, 1989
- Rapid extraction of bacterial genomic DNA with guanidium thiocyanateLetters in Applied Microbiology, 1989