AORTIC ENDOTHELIAL CELL-MIGRATION .1. MATRIX REQUIREMENTS AND COMPOSITION

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 106  (2) , 180-186
Abstract
Endothelial cell migration was studied following a mechanical injury produced in cultured confluent monolayers of calf aortic endothelium with the use of a quantitative migration assay. In this method the cells were grown on glass coverslips coated with scarlet red-containing Formvar. At confluency the cultures were cut in half with a blade; 1/2 was removed with the pigmented Formvar, and the other was returned to culture. Migration was linear for at least 96 h, and was due to cell motility, not proliferation. Since it was blocked in the presence of L-azetidine carboxylic acid of cis-hydroxyproline, inhibitors of collagen secretion, endothelial cell migration was apparently dependent on the continual secretion of collagen. The types, apparent relative amounts and localizations of the collagens as well as laminin changed during the migratory process. Aortic endothelial cell migratory response to injury is dynamic, requiring the continual secretion and modulation of matrix molecules.