Enzymic Lipid Peroxidation in the Microsomal Fraction of Rat Brain

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Abstract
An enzymic lipid peroxidation system was demonstrated in the microsomal fraction of rat brain and the requirements and optimal conditions for assay determined. The involvement of NADPH-cytochrome c reductase was demonstrated in vesicles reconstituted with lipids extracted from the brain microsomal fraction. Further characterization of the system made use of substances that inhibit the liver microsomal system. .alpha.-Tocopherol was an effective inhibitor of lipid peroxidation in the brain microsomal system; Na2SO3 had no effect, indicating that free radical transfer occurs only in the hydrophobic regions. Neither superoxide dismutase nor catalase inhibited lipid peroxidation. The implications of an NADPH-cytochrome c reductase-dependent lipid peroxidation system that is not linked to a drug hydroxylation system and appears to differ from the liver microsomal system in a number of other ways are discussed.