INVOLVEMENT OF PLASMA-MEMBRANE LIPID STRUCTURAL ORDER IN ADRIAMYCIN RESISTANCE IN CHINESE-HAMSTER LUNG-CELLS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 44  (11) , 4978-4980
Abstract
Plasma membrane preparations from Chinese hamster lung cells, which are resistant to the antitumor agent Adriamycin, were analyzed using fluorescence polarization of the membrane lipid probe trans-parinaric acid. Membranes from several drug-resistant isolates have a substantial decrease in lipid structural order relative to membranes from drug-sensitive cells. Additional studies have shown that certain isolates are unstable and undergo a sequential phenotypic reversion after continous passage in culture. Thus, cells which have reverted for membrane lipid physical changes but which still remain highly resistant to Adriamycin were identified. At later passages, these cells are found to revert to drug sensitivity. An alteration of plasma membrane lipid structural order evidently is not an essential component of the Adriamycin-resistant phenotype. In certain isolates, drug resistance and changes in membrane physical properties are both associated with an unstable genetic element.