Genetic Analysis of a High-Level Vancomycin-Resistant Isolate of Staphylococcus aureus
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- 28 November 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 302 (5650) , 1569-1571
- https://doi.org/10.1126/science.1090956
Abstract
Vancomycin is usually reserved for treatment of serious infections, including those caused by multidrug-resistant Staphylococcus aureus . A clinical isolate of S. aureus with high-level resistance to vancomycin (minimal inhibitory concentration = 1024 μg/ml) was isolated in June 2002. This isolate harbored a 57.9-kilobase multiresistance conjugative plasmid within which Tn 1546 ( vanA ) was integrated. Additional elements on the plasmid encoded resistance to trimethoprim ( dfrA ), β-lactams ( blaZ ), aminoglycosides ( aacA-aphD ), and disinfectants ( qacC ). Genetic analyses suggest that the long-anticipated transfer of vancomycin resistance to a methicillin-resistant S. aureus occurred in vivo by interspecies transfer of Tn 1546 from a co-isolate of Enterococcus faecalis .Keywords
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