Hydrogen/Deuterium Exchange Mass Spectrometry for Investigating Protein-Ligand Interactions
- 1 February 2004
- journal article
- other
- Published by Mary Ann Liebert Inc in ASSAY and Drug Development Technologies
- Vol. 2 (1) , 81-91
- https://doi.org/10.1089/154065804322966342
Abstract
Amide hydrogen/deuterium exchange mass spectrometry is rapidly becoming a powerful method for high-resolution analyses of protein dynamics, structure, and function. Hydrogen/deuterium exchange approaches can provide information that greatly augments and refines information derived from high-resolution structural studies, and can provide detailed information on native protein structure when structural information is unavailable. Application of this method for rapid analyses of protein-ligand complexes could prove useful for studies of important disease-related protein complexes. The following review covers fundamentals of hydrogen/deuterium exchange and its applications to the study of protein-ligand complexes. In addition, hydrogen/deuterium exchange mass spectrometry studies on a protein-inhibitor complex are presented.Keywords
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