Localization and Properties of Cell Wall Enzyme Activities Related to the Final Stages of Lignin Biosynthesis
- 1 March 1985
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 36 (3) , 503-510
- https://doi.org/10.1093/jxb/36.3.503
Abstract
Cell wall activities which are related to the final stages of lignin biosynthesis, that is the generation of hydrogen peroxide and peroxidase activity, were investigated using biochemical and histochemical methods. Peroxidases involved in both reactions appeared to be restricted to lignifying tissues. Isolated cell walls exhibited a very high affinity for syringaldazine. Cell walls were able, in vitro as well as in vivo, to oxidize guaiacol in the absence of H2O2 when NADH was present in the incubation medium together with p-coumarate and MnCl2 as cofactors. The origin of the NADH used to form H2O2 is discussed.This publication has 1 reference indexed in Scilit:
- Possible Multienzyme Complexes Regulating the Formation of C6-C3 Phenolic Compounds and Lignins in Higher PlantsPublished by Elsevier ,1974