Cre-mediated recombination in cell lineages that express the progesterone receptor
Top Cited Papers
- 14 February 2005
- Vol. 41 (2) , 58-66
- https://doi.org/10.1002/gene.20098
Abstract
Using gene‐targeting methods, a progesterone receptor Cre knockin (PR‐Cre) mouse was generated in which Cre recombinase was inserted into exon 1 of the PR gene. The insertion positions the Cre gene downstream (and under the specific control) of the endogenous PR promoter. As for heterozygotes for the progesterone receptor knockout (PRKO) mutation, mice heterozygous for the Cre knockin insertion are phenotypically indistinguishable from wildtype. Crossing the PR‐Cre with the ROSA26R reporter revealed that Cre excision activity is restricted to cells that express PR in progesterone‐responsive tissues such as the uterus, ovary, oviduct, pituitary gland, and mammary gland. Initial characterization of the PR‐Cre mouse underscores the utility of this model to precisely ablate floxed target genes specifically in cell lineages that express the PR. In the wider context of female reproductive tissue ontology, this model will be indispensable in tracing the developmental fate of cell lineages that descend from PR positive progenitors. genesis 41:58–66, 2005.Keywords
This publication has 14 references indexed in Scilit:
- Generation of Pax2‐Cre mice by modification of a Pax2 bacterial artificial chromosomeGenesis, 2004
- Conditional expression of oncogenic K-ras from its endogenous promoter induces a myeloproliferative diseaseJournal of Clinical Investigation, 2004
- Progesterone induction of calcitonin expression in the murine mammary glandJournal of Endocrinology, 2004
- Talking about a RevolutionDevelopmental Cell, 2004
- Defective mammary gland morphogenesis in mice lacking the progesterone receptor B isoformProceedings of the National Academy of Sciences, 2003
- A Novel LacZ Reporter Mouse Reveals Complex Regulation of the Progesterone Receptor Promoter During Mammary Gland DevelopmentMolecular Endocrinology, 2002
- Subgroup of Reproductive Functions of Progesterone Mediated by Progesterone Receptor-B IsoformScience, 2000
- Mice lacking progesterone receptor exhibit pleiotropic reproductive abnormalities.Genes & Development, 1995
- Nucleic Acid Sequence and DNase Hypersensitive Sites of the 5′ Region of the Mouse Progesterone Receptor GeneBiochemical and Biophysical Research Communications, 1994
- Derivation of completely cell culture-derived mice from early-passage embryonic stem cells.Proceedings of the National Academy of Sciences, 1993