The change in thepH of the semen of the bull after incubation

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Abstract
The mean change in thepH of 134 undiluted ejaculates of clinically normal bulls was –0·302 ± 0·027 after 1 hr. storage at 37°C. The greater thepH. change, the lower was the initialpH and the higher was the initial motility and the number of spermatozoa per c.mm. The greater the decrease inpH the poorer was the motility after incubation. The motility was better maintained in semen diluted with egg-phosphate medium, compared with undiluted semen, which agreed with the smallerpH change in this medium. There was no evidence of a relationship between thepH change and the motility after incubation and the period of time for which a high motility was maintained during storage. It is suggested that the determination of the initialpH and thepH change after 1 hr. incubation may provide a useful and accurate evaluation of the semen of the bull.

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