Common Repair Pathways Acting Upon U.V.- and X-ray Induced Damage in Diploid Cells ofSaccharomyces Cerevisiae

1 January 1984

journal article
research article
Published by Taylor & Francis in International Journal of Radiation Biology

Vol. 45 (6) , 593-606
https://doi.org/10.1080/09553008414550861

Abstract

Studies on X-ray sensitive mutants of Saccharomyces cerevisiae (Benathen 1973, Benathen and Beam 1977) show that the XS6, XS8 and XS9 genes are not only involved in the repair of X-ray-induced damage but also in the repair of U.V.-induced damage. Analysis of the U.V. sensitivity of multiple xs mutants indicates the participation of three repair pathways which differ from excision repair. Under conditions which can influence repair, such as plating of the U.V.-irradiated cells in the presence of caffeine, followed or not by hyperthermic incubation, the wild type strain shows a diphasic survival curve, consisting of an exponential component for low doses and a sigmoidal one for higher doses. Comparison with the survival curves obtained for the sensitive mutants suggests that the first component of the wild type survival curve corresponds to the inhibition of the XS6 and XS8 gene products while the appearance of a radioresistant fraction in the population relies on the induction of another repair pathway. A sequential model of repair with two branching points is proposed to explain the results.

This publication has 30 references indexed in Scilit:

Genetic control of the SOS system in E. coli
Cell, 1981
The role of dimer excision in liquid-holding recovery of UV-irradiated haploid yeast
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1980
Effect of elevated temperatures on the radiation sensitivity of yeast cells of different species
Radiation and Environmental Biophysics, 1979
Responses of radiation-sensitive mutants of Saccharomyces cerevisiae to lethal effects of bleomycin
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1978
The effect of caffeine on survival of UV-irradiated diploid yeast strains of different sensitivities
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1975
A genetic study of X-ray sensitive mutants in yeast
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1974
Synergistic interactions between rad mutations in yeast
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1973
Enhancement and Diminution of Ultraviolet‐Light‐Initiated Mutagenesis by Post‐Treatment with Caffeine in Escherichia coli
Published by Wiley ,1969
The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1968
Correspondence
The British Journal of Radiology, 1962