Artificial insemination with fresh semen in beagle bitches.

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Abstract
The intravaginal artificial insemination was carried out in bitches with fresh semen and the number of sperms and the quantity of semen required for conception was examined. Bitches were inseminated with 1 ml (group 1) or 3 ml (group 2) of semen. Each group was divided into 4 subgroups (A-D) inseminated with semen containing 2.0, 1.0, 0.5 or 0.25 .times. 108 viable sperms, respectively. The conception rates in groups a, B, C and D were 7/8 (89%), 5/15 (33%), 6/13) (46%) and 0/8 )0%), respectively, and in naturally mated group, 18/19 (95%). Groups B, C and D were significantly different in the rate of conception from the control and group A. No significant difference was observed among groups in the number of puppies born. In case of intravaginal artificial insemination with fresh semen in bitches, semen should contain 2.0 .times. 108 sperms/ml to obtain a similar rate of conception and number of offspring as in naturally breeding bitches.