High Performance Liquid Chromatographic Determination of Ginsenosides Using Photoreduction Fluorescence Detection

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Abstract
A high performance liquid chromatographic method using photoreduction fluorescence detection was described for the analysis of ginsenosides. Ginsenosides were separated on an amino column using acetonitrile and aqueous 2-tert-butylanthraquinone(t-BAQ) solution. Column effluent was passed through a 45cm-PTFE capillary tube coiled around a 10W-UV lamp to reduce t-BAQ to a highly fluorescent dihydroxy anthracene derivative which was detected by a fluorescence detector. The detection limit for the ginsenoside Rg1 by this method was found to be about 130ng. This method is less influenced by other UV-absorbing compounds compared to the conventional HPLC-UV detection method.