Intracellular stimulation of mast cells with guanine nucleotides mimic antigenic stimulation
- 25 May 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 216 (1) , 89-93
- https://doi.org/10.1016/0014-5793(87)80762-7
Abstract
Exocytosis was followed in single rat peritoneal mast cells, by measuring the cell membrane capacitance using circuit analysis and patch-clamp techniques. After antigenic stimulation or intracellular perfusion with guanine nucleotides, exocytosis followed a time course characterized by a lag period d, area expansion factor A, and a time constant τ. We suggest that A depends entirely on the cell's morphology, d reflects the properties of a GTP-binding regulatory protein that appears to rate limit the response and τ is due to an independent and yet unknown process. In contrast, cells stimulated by compound 48/80 can respond without a measurable delay and degranulate within 2 s, suggesting that this compound acts at a site after the GTP-binding regulatory protein.Keywords
This publication has 12 references indexed in Scilit:
- Interaction of retinal transducin with guanosine triphosphate analog: specificity of the .gamma.-phosphate binding regionBiochemistry, 1986
- IgE-mediated degranulation of mast cells does not require opening of ion channelsNature, 1986
- The Ca signal from fura‐2 loaded mast cells depends strongly on the method of dye‐loadingFEBS Letters, 1985
- Capacitance measurements reveal stepwise fusion events in degranulating mast cellsNature, 1984
- Islet‐activating protein, pertussis toxin, inhibits Ca2+‐induced and guanine nucleotide‐dependent releases of histamine and arachidonic acid from rat mast cellsFEBS Letters, 1984
- Geometric Parameters of Pipettes and Membrane PatchesPublished by Springer Nature ,1983
- Discrete changes of cell membrane capacitance observed under conditions of enhanced secretion in bovine adrenal chromaffin cells.Proceedings of the National Academy of Sciences, 1982
- Topology of the GTP-binding site of adenylyl cyclase from pigeon erythrocytesFEBS Letters, 1976
- Synthesis of guanosine 5'-di- and -triphosphate derivatives with modified terminal phosphates. Effect on ribosome-elongation factor G-dependent reactionsBiochemistry, 1975
- Quantitative analysis of mast cell structureJournal of Microscopy, 1974