Retrieval of leukocyte antigens in paraffin-embedded rat tissues.
- 1 July 1996
- journal article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 44 (7) , 767-776
- https://doi.org/10.1177/44.7.8675998
Abstract
We compared the immunohistochemical reactivity of various rat leukocyte antigens in frozen and paraffin-embedded thymus, spleen, abdominal lymph node, liver, and brain tissues of healthy Sprague-Dawley rats, fixed in various fixatives. Immune reactivity after fixation in Methacarn was superior to that of 4% neutral buffered formalin, a mercury-based fixative (B-5), or Carnoy. Microwave (MW) antigen retrieval (AR) enhanced antigen reactivity. Ten of the 11 leukocyte antigens studied could be retrieved in Methacarn-fixed, paraffin-embedded sections with a reactivity comparable to that obtained on frozen sections.Keywords
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