The Purification, Characterization and Partial Sequence Determination of a Trout Testis Non‐histone Protein, HMG‐T

Publisher Website
Google Scholar
Abstract
A specific non-histone chromatin-associated protein, having a high content of acidic and basic amino acids, was isolated from the chromatin of rainbow trout (Salmo gairdnerii) testis cells. The protein was prepared by the extraction of chromatin with 0.35 M NaCl and purified by chromatography on CM-cellulose with a gradient of LiCl at pH 9.0. The amino acid sequence of the first 29 residues of the N-terminal region was determined using an automatic protein sequencer. The primary structure of this protein differs from that of any of the histones yet sequenced and cannot be a degradation product of any of them. The N-terminal amino acid sequence shows considerable similarity to the HMG-1 [high mobility group] and HMG-2 chromosomal proteins described by Goodwin et al. whose N-terminal sequences were also determined in this laboratory.