Rapid method for visual identification of specific DNA sequences based on DNA-tagged liposomes
Open Access
- 1 August 1996
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 42 (8) , 1206-1209
- https://doi.org/10.1093/clinchem/42.8.1206
Abstract
We describe a rapid method for visually determining specific DNA sequences at femtomole concentrations. Liposomes, encapsulating a red dye and labeled with oligonucleotide, were used in a capillary migration-sandwich hybridization assay. Capture probe was immobilized on nitrocellulose strips, and liposomes, migrating along each strip, formed a visually discernible band in the presence of target DNA. One femtomole of synthetic target sequence could be detected in < 10 min. Sufficiently stringent hybridization conditions can be used to allow the discrimination of a 10% mismatch sequence from perfectly complementary DNA. A 366-base PCR product was detected at 200 fmol.Keywords
This publication has 0 references indexed in Scilit: