Measure of carbon and nitrogen stable isotope ratios in cultured cells
- 1 April 2004
- journal article
- Published by American Chemical Society (ACS) in Journal of the American Society for Mass Spectrometry
- Vol. 15 (4) , 478-485
- https://doi.org/10.1016/j.jasms.2003.11.019
Abstract
We report the measurement of the natural isotope ratios of nitrogen and carbon in subcellular volumes of individual cells among a population of cultured cells using a multi-isotope imaging mass spectrometer (MIMS), [MIMS is the prototype of the NanoSIMS 50, Cameca, France.] We also measured the nitrogen and carbon isotope ratio in cells after they had been cultured in media enriched with the amino acid glycine labeled with either 13C or 15N. The results demonstrate that 13C/12C and 15N/14N isotope ratios can be measured directly on a subcellular scale. This opens the way for the use of stable isotopes, in particular 15N, as labels to measure the intracellular turnover of biomolecules. Such a capability should help resolve a wide range of biomedical problems.Keywords
This publication has 17 references indexed in Scilit:
- Exploration of Inorganic C and N Assimilation by Soil Microbes with Time-of-Flight Secondary Ion Mass SpectrometryApplied and Environmental Microbiology, 2002
- Dynamic Secondary Ion Mass Spectrometry Analysis of Boron from Boron Neutron Capture Therapy Drugs in Co-Cultures: Single-Cell Imaging of Two Different Cell Types within the Same Ion Microscopy Field of ImagingAnalytical Chemistry, 2001
- Organic ion imaging of biological tissue with secondary ion mass spectrometry and matrix‐assisted laser desorption/ionizationJournal of Mass Spectrometry, 2001
- Secondary ion images of the rodent brainJournal of the American Society for Mass Spectrometry, 2000
- Peer Reviewed: A Subcellular Imaging by Dynamic SIMS Ion Microscopy.Analytical Chemistry, 2000
- SIMS MICROSCOPY: METHODOLOGY, PROBLEMS AND PERSPECTIVES IN MAPPING DRUGS AND NUCLEAR MEDICINE COMPOUNDSCell Biology International, 1997
- SIMS microscopy: a tool to measure the intracellular concentration of carbon 14‐labelled moleculesBiology of the Cell, 1992
- Mapping the cellular distribution of labelled molecules by SIMS microscopyBiology of the Cell, 1992
- Scanning secondary ion analytical microscopy with parallel detectionBiology of the Cell, 1992
- Biological microanalysis by secondary ion mass spectrometry: Status and prospectsUltramicroscopy, 1988