Effects of Time and Temperature on Digestion of Purified Antigen by Geocoris punctipes (Hemiptera: Lygaeidae) Reared on Artificial Diet
- 1 November 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Annals of the Entomological Society of America
- Vol. 83 (6) , 1177-1180
- https://doi.org/10.1093/aesa/83.6.1177
Abstract
An investigation of the effect of time and temperature on digestion using a single radial immunoassay (SRI) was undertaken. The big-eyed bug, Geocoris punctipes (Say), was used as the model insect. The insects were allowed to feed 1 h on an antigen-spiked artificial diet, then held at various time and temperature regimes. Rabbit anti-IgG was incorporated into an agarose medium for the SRI assay. The assay was sufficiently sensitive to detect rabbit IgG in insect gut extracts for at least 48 h when held at 27°C and for at least 24 h when held at 40°C.This publication has 4 references indexed in Scilit:
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