Fcε receptor mediated Ca2+ influx into mast cells is modulated by the concentration of cytosolic free Ca2+ ions
- 28 September 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 310 (2) , 123-128
- https://doi.org/10.1016/0014-5793(92)81311-9
Abstract
The relationship between the Fcε receptor mediated stimulation of mast cells and the Ca2+ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca2+ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP3-independent increase in [Ca2+]i (from resting levels of 83–150 nM to 600–680 nM), and a secretory response amounting to 30–50% of that observed upon FcεRI clustering. The TG induced rise of [Ca2+]i is most probably provided by both arrest of its uptake by the endoplasmic reticulum and influx from the medium. Thus, Ca2+ influx in mast cells may be modulated by the [Ca2+]i level.Keywords
This publication has 39 references indexed in Scilit:
- A novel cell-permeable cromoglycate derivative inhibits type I Fc.epsilon. receptor mediated calcium influx and mediator secretion in rat mucosal mast cellsBiochemistry, 1991
- ‘Quanta’ Ca2+ release and the control of Ca2+ entry by inositol phosphates ‐ a possible mechanismFEBS Letters, 1990
- IgE receptor-activated calcium permeability pathway in rat basophilic leukemia cells: measurement of the unidirectional influx of calcium using quin2-buffered cellsBiochemistry, 1987
- Synergistic signals in the mechanism of antigen-induced exocytosis in 2H3 cells: evidence for an unidentified signal required for histamine release.The Journal of cell biology, 1987
- Stimulation of superoxide anion formation by the non-TPA type tumor promoters palytoxin and thapsigargin in porcine and human neutrophilsBiochemical and Biophysical Research Communications, 1987
- Activation of Basophils and Mast Cells for Mediator ReleaseInternational Archives of Allergy and Immunology, 1987
- Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.The Journal of cell biology, 1982
- Thirteen hybridomas secreting hapten‐specific immunoglobulin E from mice with Iga or Igb heavy chain haplotypeEuropean Journal of Immunology, 1981
- IgE‐induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clonesEuropean Journal of Immunology, 1981
- Exocytosis (Secretory Granule Extrusion) Induced by Injection of Calcium into Mast CellsCanadian Journal of Physiology and Pharmacology, 1973