The spectrophotometric determination of vitamin D in fresh-water fish liver oils

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Abstract
There are many difficulties associated with the determination of vitamin D, especially in natural products such as fish liver oils. Vitamin A is the chief interfering material; it masks the absorption of vitamin D both in the ultraviolet region and in the antimony trichloride colour test, making the determination of vitamin D almost impossible. In addition to vitamin A1, fresh-water fish liver oils contain vitamin A2, which also interferes in direct spectrophotometry. A method for determining vitamin D is described, in which vitamins A1 and A2 are eliminated by converting them to anhydro-vitamins A1 and A2 and separating them from vitamin D by chromatography on an alumina column.

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