Preparation of highly potent intrinsic factor mucoprotein

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Abstract
A mucoprotein was obtained from dried pig-stomach powder by a technique involving ammonium sulfate precipitation, chromatography on a column of IRC50 and fractionation with cold ethanol. It was homogeneous after paper-strip electrophoresis at pH 6.4 and 8.0, and the fraction from which it was prepared was almost homogeneous in the ultracentrifuge. The mucoprotein would appear to have a molecular weight of approximately 15,000. A dose of 0.5 mg proved effective in the fecal-excretion test with radioactive cyanocobalamin.