Release of Amphotericin B from Delivery Systems and its Action Against Fungal and Mammalian Cells
- 1 January 1997
- journal article
- Published by Taylor & Francis in Journal of Drug Targeting
- Vol. 4 (5) , 311-319
- https://doi.org/10.3109/10611869708995847
Abstract
Spectroscopic studies of four amphotericin B (AmB) lipid preparations--small negatively charged unilamellar vesicles "AmBisome", positively charged oligolamellar liposomes "Ampholiposomes", AmB Lipid Complex "L-AmpB33"--and AmB association with gamma cyclodextrin demonstrated that the composition of drug delivery system directly influences AmB organization. The aggregation state and release in external medium of AmB was monitored by circular dichroism and UV-visible absorption. AmB short-term activity against Candida albicans (K+ leakage) was found to be correlated with the amount of free AmB released from lipid preparations. These data seem to indicate that lipid composition influences anti-Candida albicans activity, by modulation of AmB binding to lipids.Keywords
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