RNase T1-resistant oligonucleotides of B-tropic murine leukemia virus from BALB/c and five of its NB-tropic derivatives

Publisher Website
Google Scholar
Abstract
Two-dimensional gel electrophoresis was used to obtain fingerprints of RNase T1-resistant oligonucleotides of a B-tropic murine leukemia virus from BALB/c [mouse] and 5 NB-tropic viruses independently derived from this B virus by passage through NIH Swiss mouse embryo cells in vitro. The fingerprints of the B- and NB-tropic viruses were very similar: about 33 of 35 large T1-resistant oligonucleotides appeared to be shared by these viruses. The 5 NB-tropic viruses possessed an apparently common alteration relative to their B virus progenitor. This change involved the acquistion of 1 oligonucleotide and, tentatively, the loss of 1 oligonucleotide. These changes may represent an alteration responsible for the change from B- to NB-tropism. Fingerprints of B- and NB-tropic viruses were not affected when the viruses were grown in cells of different Fv-1 type.