Distinctive Electrophoretic and Isoelectric Focusing Patterns of Esterases from Yersinia enterocolitica and Yersinia pseudotuberculosis

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Abstract
Esterases of 53 strains of Y. enterocolitica s.s., including 5 previously defined biotypes, and 30 strains of Y. pseudotuberculosis were analyzed by horizontal polyacrylamide-agarose gel electrophoresis and by isoelectrofocusing in thin-layer polyacrylamide gel. Esterase bands were defined by their range of activity towards several synthetic substrates and their resistance to heat and to DFP. The 2 spp. were characterized by distinct electrophoretic patterns of their esterases. The apparent MW of the heat-resistant esterase of Y. enterocolitica and of the major heat-resistant esterase of Y. pseudotuberculosis, as determined by polyacryalmide gradient gel electrophoresis, were estimated to be 52,000 nd 250,000, respectively. On the basis of electrophoretic mobilities and isoelectric points of esterases produced by strains of Y. enterocolitica, 5 principal zymotypes were observed: 2 for strains of biotype 1, 2 for strains of biotypes 2 and 3, respectively, and only 1 for strains of both biotypes 4 and 5. The zymotypes of strains of biotypes 2, 3, 4 and 5 were more closely related to one another than to zymotypes of strains of biotype 1. Variations in number or mobility of bands observed within each biotype of Y. enterocolitica and within some serotypes of Y. pseudotuberculosis could represent an additional marker for epidemiological analysis.

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