Monoclonal antibodies specific for African swine fever virus proteins

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Abstract
Sixty stable hybridomas which produced Ig that recognized 12 proteins from African swine fever virus particles and African swine fever virus-infected porcine alveolar macrophage cells were obtained. Most of the monoclonal antibodies were specific for the 3 major structural proteins p150, p72 and p12. The specificity of some monoclonal antibodies for the structural proteins p150 and p37 and the nonstructural proteins p220 and p60 indicated that proteins p150 and p220 are antigenically related to proteins p37 and p60. The association of some viral antigens to specific subcellular components was determined by immunofluorescence and analysis of the binding of monoclonal antibodies to infected cells. A host protein (p24) seemed to be associated with the virus particles.