Effects of Hormone Dose and Length of Injection on Mouse Mammary Gland Growth.

Abstract
Male mice were fed diethylstilbestrol for 4 weeks to insure duct growth. In addition, one group was injected subcutaneously with 0.75 [mu]g estradiol benzoate (EB/day along with various levels of progesterone (P) for 5- or 10-day periods. Another group received 0.50 [mu]g EB/day plus various amounts of P for a 10-day period. Desoxyribosenucleic acid was used as a growth index. Results indicated that best slope with greatest precision was obtained when 0.75 [mu]g EB/day with various amounts of progesterone was injected for 10-day period.