ASCARIS AND EOSINOPHIL .2. ISOLATION AND CHARACTERIZATION OF EOSINOPHIL CHEMOTACTIC FACTOR AND NEUTROPHIL CHEMOTACTIC FACTOR OF PARASITE IN ASCARIS ANTIGEN

  • 1 January 1979
    • journal article
    • research article
    • Vol. 122  (1) , 302-308
Abstract
An eosinophil chemotactic factor of the parasite (ECF-P) was found in extracts from the body cavity fluid of the Ascaris species. The physicochemical characteristics of ECF-P were determined to elucidate the relationship between ECF-P and Ascaris antigens [Ag]. ECF-P was active in in vivo and in vitro chemotactic assay systems and was heat labile, nondialyzable, and stable to lyophilization. The estimated MW was approximately 30,000 daltons. The isoelectric point of ECF-P was 8.5. It was quite different from the Ascaris Ag in the same extract, or various Ascaris allergens reported previously by other researchers. ECF-P did not show any activity in eliciting a guinea pig passive cutaneous anaphylaxis (PCA) reaction. ECF-P is not identical to Ascaris Ag. The neutrophil chemotactic factor of the parasite (NCF-P), with at least 2 components, is present in the Ascaris extract, and is heat labile and resistant to dialysis and lyophilization, but is separable from coexistent ECF-P due to their different physicochemical properties.

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