An altered oxidant defense system in red blood cells affects their ability to release nitric oxide-stimulating ATP
- 19 May 2006
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Molecular BioSystems
- Vol. 2 (6-7) , 305-311
- https://doi.org/10.1039/b604362n
Abstract
A novel microflow technique is used to demonstrate that a weakened oxidant defense system found in diabetic erythrocytes leads to decreased levels of deformation-induced release of adenosine triphosphate (ATP) from erythrocytes. Addition of an oxidant to rabbit erythrocytes resulted in a 63% decrease in deformation-induced ATP release before eventually recovering to a value that was statistically equivalent to the initial value. Inhibition of glucose-6-phosphate dehydrogenase prevents recovery from the oxidant attack. Finally, results indicated that the ATP release from the erythrocytes of type II diabetics (91 nM ± 10 nM) was less than half of that measured from the erythrocytes of healthy controls (190 ± 10 nM). These data suggest that the antioxidant status of erythrocytes is a critical determinant in the ability of these cells to release ATP, a known nitric oxide stimulus.Keywords
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