Determination of Glucose in Biologic Fluids with an Automated Enzymatic Procedure
- 1 September 1965
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 11 (9) , 840-845
- https://doi.org/10.1093/clinchem/11.9.840
Abstract
An automated procedure for the enzymatic determination of "true" glucose with the glucose oxidase-peroxidase system is described. A threefold increase in sensitivity was obtained through measurement of the stable, pink chromogen obtained at high acid concentration. Good reproducibility and stoichiometry was obtained over the range of 50-400 mg./l00 ml. at a sampling rate of 40/hr. The normal range (70-105 mg./100 ml.) and the error of the method (±2.0%) are about the same as that obtained with the manual method of Saifer and Gerstenfeld (7).Keywords
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