THE LOCATION OF [6,7-3H]OESTRADIOL-17β IN MOUSE VAGINA
- 1 June 1968
- journal article
- research article
- Published by Bioscientifica in Journal of Endocrinology
- Vol. 41 (2) , 223-229
- https://doi.org/10.1677/joe.0.0410223
Abstract
SUMMARY The retention of [6,7-3H]oestradiol-17β in fractions of the mouse vagina has been studied after direct application of 50 pg. of this oestrogen. Tritium was present in both epithelial and stromal cells. Most of the tritium was associated with the nuclear fraction from the epithelium. The nuclear binding was acid labile and only 30% of the nuclear tritium was extracted with 2 m-NaCl. The results obtained by sonic disruption of the nuclei were compatible with the view that the [6,7-3H]oestradiol-17β was associated with chromatin. No evidence was obtained for the presence of a cytoplasmic oestradiol-binding component in the epithelium.This publication has 1 reference indexed in Scilit:
- Stereospecific binding of estrogens in the rat uterusArchives of Biochemistry and Biophysics, 1965