Interactions between human cyclin T, Tat, and the transactivation response element (TAR) are disrupted by a cysteine to tyrosine substitution found in mouse cyclin T
Open Access
- 16 February 1999
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 96 (4) , 1285-1290
- https://doi.org/10.1073/pnas.96.4.1285
Abstract
The transcriptional transactivator Tat from HIV binds to the transactivation response element (TAR) RNA to increase rates of elongation of viral transcription. Human cyclin T supports these interactions between Tat and TAR. In this study, we report the sequence of mouse cyclin T and identify the residues from positions 1 to 281 in human cyclin T that bind to Tat and TAR. Mouse cyclin T binds to Tat weakly and is unable to facilitate interactions between Tat and TAR. Reciprocal exchanges of the cysteine and tyrosine at position 261 in human and mouse cyclin T proteins also render human cyclin T inactive and mouse cyclin T active. These findings reveal the molecular basis for the restriction of Tat transactivation in rodent cells.Keywords
This publication has 17 references indexed in Scilit:
- Transcription elongation factor P-TEFb mediates Tat activation of HIV-1 transcription at multiple stagesThe EMBO Journal, 1998
- HIV-1: Fifteen Proteins and an RNAAnnual Review of Biochemistry, 1998
- Requirements for RNA Polymerase II Carboxyl-terminal Domain for Activated Transcription of Human Retroviruses Human T-Cell Lymphotropic Virus I and HIV-1Published by Elsevier ,1996
- Trans-activation by human immunodeficiency virus Tat protein requires the C-terminal domain of RNA polymerase II.Proceedings of the National Academy of Sciences, 1996
- Control of RNA Polymerase II Elongation Potential by a Novel Carboxyl-terminal Domain KinaseJournal of Biological Chemistry, 1996
- CONTROL OF RNA INITIATION AND ELONGATION AT THE HIV-1 PROMOTERAnnual Review of Biochemistry, 1994
- Specific Interaction of the Human Immunodeficiency Virus Tat Proteins with a Cellular Protein KinaseVirology, 1993
- Human Chromosome 12 Is Required for Elevated HIV-1 Expression in Human-Hamster Hybrid CellsScience, 1989
- The trans-activator gene of the human T cell lymphotropic virus type III is required for replicationCell, 1986
- The trans-activator gene of HTLV-III is essential for virus replicationNature, 1986