Deacetylation of PS-5, a new .BETA.-lactam compound. I. Microbial deacetylation of PS-5.

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Abstract
PS 5 was deacetylated to NS 5 (deacetylated PS 5) by L-amino acid acylase from porcine kidney and D-amino acid acylase from Streptomyces olivaceus but not by L-amino acid acylase from Aspergillus sp. Using PS 5, N-chloroacetyl-L-phenylalanine and N-chloroacetyl-D-valine as substrates, acylase producers were screened among facultative methanol-assimilating bacteria. Most of the microbes tested were active and could be classified into 2 groups of L-acylase producers and L- and D-acylase producers. Pseudomonas sp. 1158 which deacetylated the 3 substrates was chosen for further study. Cells of the bacterium entrapped in polyacrylamide gel and its acylase activities immobilized on DEAE-Sephadex were useful for conversion of PS5 to NS5.