A Substance Effecting Differentiation in Streptomyces griseus

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Abstract
A conidium-producing variant of S. griseus, strain 45-H, produces a substance, factor C, which is capable of inducing conidium formation in the hyphae of a conidium-non-producing mutant, strain 52-1. Factor C can be determined quantitatively on the basis of this biological effect. The biologically active substance can be purified by ion-exchange chromatography on cellulose phosphate combined with affinity chromatography on DNA-agarose. The purified substance is concentrated at least 1700 times. The MW of factor C, estimated by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, is about 34,500. On determining the amino acid composition of factor C, 60% of the amino acids were hydrophobic.