In vitro translation products specified by the transforming region of adenovirus type 2

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Abstract
Region 1 DNA sequences (map positions 0-11% on the linear adenovirus 2 genome) are expressed early and late in lytic infection and are required for transformation by the virus. During productive infection 6 distinct cytoplasmic RNA are synthesized from this region. These RNA comprise 2 families, each consisting of 3 size classes that share 3'' sequences. Region 1 RNA were purified by hybridization selection using restriction fragments bound to nitrocellulose membranes and by size fractionation. The isolated RNA were translated in cell-free systems derived from wheat germ and rabbit reticulocytes. The family of RNA specified by 0-4.4 sequences includes 2 RNA which are 12S and 13S in size. These RNA were partially separated by MW and translated. The 13S RNA produced 53,000 dalton (53K) and 41K peptides, and the 12S RNA synthesized 47K and 35K products. The family of RNA mapping from 4.4-11.0 encodes 3 separate polypeptides, each of which can be assigned to a specific RNA. A 12K product that co-migrates with structural polypeptide IX is synthesized from the 9S RNA. The 13S RNA encodes a 15K polypeptide that corresponds to a 15K polypeptide in infected cell extracts. The 22S RNA encodes a 52K protein distinct from the 0-4.4 polypeptides.