Abstract
An improved method for the purification of hepatic proliferation inhibitor from rat liver by means of anion-exchange HPLC has been developed. The inhibitor can be purified on an anion exchange HPLC column by using a linear sodium phosphate gradient. The HPLC method allows repeated use of one column and is both rapid and reproducible. The hepatic proliferation inhibitor isolated by this method retains all of its biological activity and is homogeneous as revealed by reverse-phase HPLC.