Preparation of Succinimidyl and Pentafluorophenyl Active Esters of 5- and 6- Carboxyfluorescein

1 March 1997

journal article
Published by American Chemical Society (ACS) in Bioconjugate Chemistry

Vol. 8 (2) , 253-255
https://doi.org/10.1021/bc9600877

Abstract

A mixture of 5- and 6-carboxyfluorescein was activated with 1-[3-(dimethylamino)propyl]-3-ethylcarbodiimide hydrochloride in the presence of either N-hydroxysuccinimide or pentafluorophenol to give the corresponding succinimidyl and pentafluorophenyl esters. The regioisomeric mixtures were separated to give the 5- and 6- succinimidyl and pentafluorophenyl active esters in >98% purity.

Keywords

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